French NCI (INCa) Molecular Biology Platform of the FHU ONCOAGE

Activity area(s)

The two laboratories (molecular pathology unit of LPCE Pasteur Hospital and molecular biology unit of the pharmacogenomics laboratory at the CLCC CAL) are integrated in the Molecular Genetic PACA East Platform labelled by the French NCI since 2006. Moreover these two laboratories are accredited according the ISO 15189 norm. The molecular pathology units joined their efforts to work mainly in lung diseases pathology and in head and neck pathology (including thyroid cancer).


The platform is equipped with an ABI 3500, a Sequenom Mass Arrays, an Agilent Scanner, a PGM ion Torrent, different pyrosequencers a Rotorgen, etc. Molecular biology approaches are made from plasma, cytological and tissue samples. Different projects concern the assessment of DNA and microRNA signatures from lung cancers. Moreover the development of NGS with different panel of genes of interest was set up since two years and the main objective in now to be accredited according to the ISO15189 norm by the first months of 2016.


Gérard Milano & Paul Hofman

Type of structure

Nice Hospital and Comprehensive Cancer Center Centre Antoine Lacassagne

Recognition by

French NCI (INCa)


Pasteur Hospital Pavillon J 30 avenue de la voie romaine Nice and CLCC CAL avenue de valombrose Nice

Contact name

Paul Hofman Gérard Milano

Contact mail,

Skills of the team members

  • Long Elodie
  • Ilie Marius
  • Hofman Véronique
  • Lassalle Sandra
  • Lespinet Virginie
  • Bordone Olivier
  • Ribeyre CamilleEbran Nathalie
  • Hofman Paul
  • Milano Gérard Molecular Pathologist, MD, PhD
  • Molecular Pathologist, MD, PhD, HDR
  • Molecular Pathologist, MD, PhD
  • Molecular Pathologist, MD, PhD
  • Ingenior Molecular Pathology, PhD
  • Ingenior Molecular Pathology, MSc
  • Ingenior Bioinformatic, MSc
  • Ingenior Molecular Pathology, PhD
  • Molecular pathologist, MD, PhD
  • Pharmacologist, PhD

Five top/relevant papers in the last five years related to the platform activity

Lantuejoul S, Rouquette I, Blons H, Le Stang N, Ilie M, Begueret H, Grégoire V, Hofman P, Gros A, Garcia S, Monhoven N, Devouassoux-Shisheboran M, Mansuet-Lupo A, Thivolet F, Antoine M, Vignaud JM, Penault-Llorca F, Galateau-Sallé F, McLeer-Florin A. French multicentric validation of ALK rearrangement diagnostic in 547 lung adenocarcinomas. Eur Respir J. 2015 Jul;46(1):207-18

Long E, Hofman V, Ilie M, Washetine K, Lespinet V, Bonnetaud C, Bordone O, Gavric-Tanga V, Gaziello MC, Lassalle S, Selva E, Zahaf K, Lamy A, Sabourin JC, Hofman P. Accreditation of the activity of molecular pathology according to ISO 15189: key steps to follow and the main potential pitfalls. Ann Pathol. 2013 Feb;33(1):12-23.

Ilie M, Hofman P. Pitfalls in lung cancer molecular pathology: how to limit them in routine practice? Curr Med Chem. 2012;19(16):2638-51

Thariat J, Vignot S, Lapierre A, Falk AT, Guigay J, Van Obberghen-Schilling E, Milano G. Integrating genomics in head and neck cancer treatment: Promises and pitfalls. Crit Rev Oncol HematolApr 18. pii: S1040-8428(15)00061-X. doi: 10.1016/j.critrevonc.2015.03.005

Etienne-Grimaldi MC, Mahamat A, Chazal M, Laurent-Puig P, Olschwang S, Gaub MP, Formento JL, Formento P, Sudaka A, Boige V, Abderrahim-Ferkoune A, Benchimol D, André T, Houry S, Faucheron JL, Letoublon C, Gilly FN, Delpero JR, Lasser P, Pradere B, Pezet D, Penault-Llorca F, Milano G. Molecular patterns in deficient mismatch repair colorectal tumours: results from a French prospective multicentric biological and genetic study. Br J Cancer. 2014 May 27;110(11):2728-37

Contribution to the FHU